Journal: Research in Veterinary Science

Article Title: Relationship between rate of infection and markers of inflammation/immunity in Holy Birman cats with feline coronavirus

doi: 10.1016/j.rvsc.2014.08.009

Figure Lengend Snippet: Results recorded in Birman cats and in cats from other breeds.

Article Snippet: Immunophenotyping by flow cytometry was performed on aliquots of 50 μL of the cell suspension to identify lymphocyte subpopulations as previously described ( ) using the following panel of antibodies for feline surface antigen: 2.5 μL of mouse anti feline CD4 (specific for T helper cells, clone MCA1350, Serotec, Oxford, UK), 1 μL of mouse anti feline CD8 alpha/beta (specific for T cytotoxic cells, clone MCA1347G, Serotec, Oxford, UK), 50 μL of mouse anti feline CD5 (specific for T cells, clone MCA2038S, Serotec, Oxford, UK), and 1 μL of mouse anti canine CD21, specific for B cells, that cross reacts with feline species (clone MCA1781R, Serotec).

Techniques:

Journal: Journal of leukocyte biology

Article Title: CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.

doi: 10.1189/jlb.0408255

Figure Lengend Snippet: Fig. 2. Morphology of CD5lo and CD5hi cells after IL-2 stimulation; May-Grunwald Giemsa stain. PBMCs were stained using anti-CD5 antibody and sorted into CD5lo and CD5hi populations using a FACSAriaTM flow cytometer. Sorted CD5lo (a) and CD5hi (b) cells were cultured with IL-2 (2500 IU/ml) for 6 days. The IL-2-stimulated CD5hi and CD5lo cells were smeared on a glass slide, allowed to air dry, and stained with May-Grunwald Giemsa stain. Each cell preparation was examined by light microscopy (phase contrast, 1000). The original bars represent 10 m. The diameters of IL-2-stimulated CD5lo and CD5hi cells were measured by Image-Pro Plus software and are present in c. (c) The data were collected from six different dogs and analyzed by a t-test. *, P 0.05; **, P 0.01.

Article Snippet: Briefly, for direct immunofluorescence analysis, cells were incubated with FITC-conjugated isotype control or FITC-conjugated rat anti-canine CD5 mAb (Serotec, Oxford, UK).

Techniques: Giemsa Stain, Staining, Cytometry, Cell Culture, Light Microscopy, Software

Journal: Journal of leukocyte biology

Article Title: CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells.

doi: 10.1189/jlb.0408255

Figure Lengend Snippet: Fig. 6. IFN- production by CD5lo and CD5hi cells following IL-12 stimula- tion. IFN- production by CD5lo and CD5hi cells was measured by intracellular staining with an anti-IFN- antibody with a Becton Dickinson Cytofix/Cyto- perm Plus kit and GolgiStop. Canine PBMC were stimulated for 72 h with 5 ng/ml rcIL-12. Cells were labeled by FITC-conjugated anti-canine CD5 mAb, gated for CD5lo or CD5hi cells, respectively, and then stained with anti-IFN- -PE for intracellular visualization of IFN- (a). For another experiment, CD5lo

Article Snippet: Briefly, for direct immunofluorescence analysis, cells were incubated with FITC-conjugated isotype control or FITC-conjugated rat anti-canine CD5 mAb (Serotec, Oxford, UK).

Techniques: Staining, Labeling